piggyBac Transposon System Modification of Primary Human T Cells
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10:02 min
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November 5th, 2012
DOI :
November 5th, 2012
•Sunandan Saha1,2, Yozo Nakazawa3, Leslie E. Huye4,5, Joseph E. Doherty2,6, Daniel L. Galvan2, Cliona M. Rooney4,5,7, Matthew H. Wilson1,2,4,8
1Program in Translational Biology and Molecular Medicine, Baylor College of Medicine , 2Department of Medicine, Division of Nephrology, Baylor College of Medicine , 3Department of Immunology and Pathology, Shinshu University School of Medicine, 4Center for Cell and Gene Therapy, Baylor College of Medicine , 5Department of Pediatrics, Baylor College of Medicine , 6Program in Cell and Molecular Biology, Baylor College of Medicine , 7Department of Molecular Virology and Microbiology, Baylor College of Medicine , 8Michael E. DeBakey VA Medical Center
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0:05
Title
1:39
Day 0: Isolation of PBMCs from Human Blood
3:23
Day 1: Coating Plates with anti-CD28 and anti-CD3 Antibody for Stimulating T Cells
3:59
Day 1: Nucleofection of Unstimulated T Cells
5:54
Day 2: Nonspecific Stimulation of T Cells
7:28
Day 7: Analysis of Gene Expression
8:05
Results: piggyBac-mediated Gene Modification of Human T Cells
9:19
Conclusion
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