Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
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11:47 min
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February 8th, 2013
DOI :
February 8th, 2013
•Rebecca E. Nakles1, Sarah L. Millman1, M. Carla Cabrera1, Peter Johnson1,2, Susette Mueller1,2, Philipp S. Hoppe3, Timm Schroeder3, Priscilla A. Furth1,2,4,5
1Department of Oncology, Georgetown University, 2Lombardi Comprehensive Cancer Center, Georgetown University, 3Stem Cell Dynamics, Helmholtz Zentrum München - German Research Center for Environmental Health, 4Department of Medicine, Georgetown University, 5Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science, Dankook University
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0:05
Title
1:48
Generation of Primary Mammary Epithelial Cell Cultures
3:39
Live-cell Imaging
5:56
Assessing Cellular Event from Time-lapse Images
7:50
Making Single Cell Fate Maps with Timm's Tracking Tool (TTT)
9:59
Representative Results: Colony Formation in BRCA1 Disrupted Cells
11:12
Conclusion
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