Purification of Transcripts and Metabolites from Drosophila Heads
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12:49 min
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March 15th, 2013
DOI :
March 15th, 2013
•Kurt Jensen1, Jonatan Sanchez-Garcia1, Caroline Williams2, Swati Khare1, Krishanu Mathur1, Rita M. Graze3, Daniel A. Hahn2, Lauren M. McIntyre3, Diego E. Rincon-Limas1,4, Pedro Fernandez-Funez1,4
1Department of Neurology, McKnight Brain Institute, University of Florida , 2Department of Entomology and Nematology, University of Florida , 3Genetics Institute, Department of Molecular Genetics and Microbiology, University of Florida , 4McKnight Brain Institute, Department of Neuroscience, Genetics Institute, Center for Translational Research on Neurodegenerative Diseases, and Center for Movement Disorders and Neurorestoration, University of Florida
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0:05
Title
1:24
Generating a Replicate and Freezing Flies
2:42
Collection and Lyophilization of Fly Heads
3:53
RNA-specific Procedures: Total RNA Extraction and mRNA Purification
7:23
Metabolite-specific Procedures: Methanol-chloroform Extraction
9:54
Representative Results
12:23
Conclusion
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