Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

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13:43 min

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June 24th, 2013

DOI :

10.3791/50386-v

June 24th, 2013

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Sangmi Jun¹, Gongpu Zhao¹, Jiying Ning¹, Gregory A. Gibson², Simon C. Watkins², Peijun Zhang¹

¹Department of Structural Biology, University of Pittsburgh School of Medicine, ²Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine

Резюме

We describe a correlative microscopy method that combines high-speed 3D live-cell fluorescent light microscopy and high-resolution cryo-electron tomography. We demonstrate the capability of the correlative method by imaging dynamic, small HIV-1 particles interacting with host HeLa cells.

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Correlative Microscopy

Cryo electron Tomography

Live cell Imaging

HIV 1

Virus host Interactions

3D Structural Analysis

Fluorescence Microscopy

Cryo fluorescence Microscopy

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