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JoVE Journal

Medicine

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Parabiosis in Mice: A Detailed Protocol

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73.9K Views

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07:56 min

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October 6th, 2013

DOI :

10.3791/50556-v

October 6th, 2013

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Paniz Kamran¹^,², Konstantina-Ioanna Sereti¹^,², Peng Zhao¹^,², Shah R. Ali³, Irving L. Weissman³, Reza Ardehali¹^,²

¹Department of Medicine-Division of Cardiology, University of California, Los Angeles, ²Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, ³Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine

Резюме

Parabiotic joining of two organisms leads to the development of a shared circulatory system. In this protocol, we describe the surgical steps to form a parabiotic connection between a wild-type mouse and a constitutive GFP-expressing mouse.

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