<h2 class="jove_title"><a href="https://www.jove.com/video/50525">Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy</a></h2>
Lisa Gfrerer1, Max Dougherty1, Eric C. Liao1
1<a href='/institutions/NA-north-america/US-united-states/MA-massachusetts/24866-massachusetts-general-hospital'>Center for Regenerative Medicine, Massachusetts General Hospital</a>
Visualization of experimental data has become a key element in presenting results to the scientific community. Generation of live time-lapse recording of growing embryos contributes to better presentation and understanding of complex developmental processes. This protocol is a step-by-step guide to cell labeling via photoconversion of kaede protein in zebrafish.
<h2 class="jove_title"><a href="https://www.jove.com/video/50582">Operation of a Benchtop Bioreactor</a></h2>
Kristina M. Obom1, Andrew Magno2, Patrick J. Cummings1
1Center for Biotechnology Education, Krieger School of Arts and Sciences, Johns Hopkins University, 2ATR Biotech
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture's biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
<h2 class="jove_title"><a href="https://www.jove.com/video/50604">Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion</a></h2>
Grady E. Carlson1, Eric W. Martin2, Monica M. Burdick1,2
1Department of Chemical and Biomolecular Engineering, Russ College of Engineering and Technology, Ohio University, 2Biomedical Engineering Program, Ohio University
Dual camera emission splitting systems for two-color fluorescence microscopy generate real-time image sequences with exceptional optical and temporal resolution, a requirement of certain live cell assays including parallel plate flow chamber adhesion assays. When software is employed to merge images from simultaneously acquired emission channels, pseudocolored image sequences are produced.
<h2 class="jove_title"><a href="https://www.jove.com/video/50757">Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry</a></h2>
Evan L. Pannkuk1, Thomas S. Risch2, Brett J. Savary3
1Graduate Program of Environmental Science, Arkansas State University, 2Department of Biological Sciences, Arkansas State University, 3Arkansas Biosciences Institute and College of Agriculture and Technology, Arkansas State University
Mammalian integument contains solvent-extractable lipids that can provide chemical compositions characteristic of individual species. This paper presents a routine method for separating broad lipid classes isolated from integumentary tissues using thin layer chromatography and determining the triacylglyceride profile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

september 2013 - this month in jove

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/79">September 2013 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
Palatogenesis (palate formation) involves complex developmental processes that are highly regulated and conserved among vertebrates. Thus, scientists use laboratory models like the zebrafish to study cleft palate and related developmental malformations. <a href="http://www.jove.com/video/50525">Gfrerer et al.</a> examine zebrafish embryos using time-lapse confocal microscopy, which lets them observe the migration of cranial neural crest cells (CNCCs) from the dorsal neural tube to form craniofacial structures. This method also allows live imaging of abnormal craniofacial development in zebrafish, which is used to model conditions like cleft palate in humans.
In the laboratory, bacteria, yeast, and many other cell types are commonly grown in shake flasks. However, this classic technique has many drawbacks that can prevent optimal growth, such as fluctuations in pH, oxygen concentration, nutrients, and temperature. In <a href="http://www.jove.com/bioengineering">JoVE Bioengineering</a>, <a href="http://www.jove.com/video/50582">Obom et al.</a> demonstrate a benchtop bioreactor complete with a pH probe an oxygen probe, reagent feed bottle to replenish media, precise temperature controls, a gas infuser, and an impeller that stirs the culture. This system allows optimal culture conditions for the large-scale growth of recombinant expression systems.
Also in <a href="http://www.jove.com/bioengineering">JoVE Bioengineering</a>, we have a method for live cell imaging in a flow-based cell adhesion assay. But unlike standard protocols that use single camera systems, our featured method is a dual camera emission splitting system that captures real-time images in two fluorescence channels at the same time. <a href="http://www.jove.com/video/50604">Carlson et al.</a> use a parallel plate flow chamber adhesion assay to demonstrate this dual camera system, which they use to study adhesion, rolling, and other cell behaviors.
<a href="http://www.jove.com/chem">JoVE Chemistry</a> features a not-so-common animal model: the Eastern red bat, which is widespread in the United States; however, in recent years, a fungal disease called white nose syndrome (WNS) has killed millions of these bats. WNS is a major threat to the agricultural industry, which relies on bats as a form of natural pest control. Like other mammals, bats have sebaceous glands in their skin that secrete sebum, an oily material that helps protect against infections. <a href="http://www.jove.com/video/50757">Pannkuk et al.</a> demonstrate a method for isolating the sebum from the hair and the skin of the bat, then analyze the lipid composition of the sebum using thin-layer chromatography (TLC) combined with matrix-assisted laser desorption/ionization time-of-flight (MALTI-TOF) mass spectrometry. The lipid profile of the sebum can affect immune processes and can also serve as disease markers; thus, this method will yield vital information for disease treatment and prevention in the Eastern red bat as well as other mammals.
You&#8217;ve just had a sneak peek of a few highlights from the <a href="http://www.jove.com/archive/79">September 2013 issue</a> of JoVE. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about September 2013 - This Month in JoVE at JoVE.com

September 2013 - This Month in JoVE

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/79">September 2013 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Here's a look at what's coming up in the September 2013 issue of JoVE: The Journal of Visualized Experiments.
Palatogenesis (palate formation) involves ...

Without Section

2.5K Views.  Massachusetts Eye and Ear. Here's a look at what's coming up in the September 2013 issue of JoVE: The Journal of Visualized Experiments.

Video: September 2013 - This Month in JoVE

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...