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High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

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13.7K Views

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11:34 min

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May 5th, 2014

DOI :

10.3791/51222-v

May 5th, 2014

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Marianne Lucas-Hourani¹, Hélène Munier-Lehmann², Olivier Helynck², Anastassia Komarova¹, Philippe Desprès³, Frédéric Tangy¹, Pierre-Olivier Vidalain¹

¹Unité de Génomique Virale et Vaccination, Virology Department, Institut Pasteur, CNRS UMR3569, ²Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department, Institut Pasteur, CNRS UMR3523, ³Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department, Institut Pasteur

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High throughput Screening

Broad spectrum Chemical Inhibitors

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0:05

Title

1:34

Preparation of 96-well Daughter Plates with Compounds

3:03

Preparation of Cell Cultures to Determine Compound Toxicity

4:18

Preparation of Cell Cultures Infected by rMV2/Luc

5:37

Luciferase Activity Measurement

6:36

Secondary Screening for Toxicity and Broad-spectrum Antiviral Activity

8:52

Results: Identification of Anti-viral Hit Compounds

11:06

Conclusion

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