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Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

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14:14 min

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February 28th, 2014

DOI :

10.3791/51248-v

February 28th, 2014

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Lori E. Lowes¹^,², Benjamin D. Hedley³, Michael Keeney³^,⁴, Alison L. Allan¹^,²^,⁴^,⁵

¹London Regional Cancer Program, London Health Sciences Centre, ²Department of Anatomy & Cell Biology, Schulich School of Medicine and Dentistry, Western University, ³Special Hematology/Flow Cytometry, London Health Sciences Centre, ⁴Lawson Health Research Institute, ⁵Department of Oncology, Western University

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Semiautomated CTC Assay

Circulating Tumor Cells

Preclinical Mouse Models

Metastasis

CTC Misclassification

Главы в этом видео

0:05

Title

1:42

Human Blood Sample Preparation and Processing

3:25

Sample Scanning on the Analysis Instrument

4:58

CTC Characterization for User-defined Markers Using the CSS

6:08

Adaptation of the Standard CSS Protocol: Preclinical Mouse Models

7:42

CTC Staining

9:20

Scanning of Manually Separated Samples

10:13

Results: CTC Assays in Clinical and Preclinical Research

13:43

Conclusion

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