Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
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08:14 min
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May 1st, 2014
DOI :
May 1st, 2014
•Miquella G. Chavez1,2, Jimmy Hu1, Kerstin Seidel1, Chunying Li1,3, Andrew Jheon1, Adrien Naveau4,5,6, Orapin Horst1,7, Ophir D. Klein1,8
1Department of Orofacial Sciences and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, 2Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 3Department of Pathology and Research Center, Zhongshan Hospital of Dalian University, 4Université Paris Descartes, Sorbonne Paris Cite, UMR S872, 5Centre de Recherche des Cordeliers, Université Pierre et Marie Curie, UMR S872, 6INSERM U872, 7Division of Endodontics, Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, 8Department of Pediatrics and Institute for Human Genetics, University of California, San Francisco
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0:05
Title
1:06
Dissect Lower Hemi-mandibles from Adult Mouse
2:09
Incisors Isolation
3:51
Microdissection of the Labial CL
5:31
Primary Culture and Passage of Dental Epithelial Stem Cells
6:55
Results: Isolation and Culture of DESCs from the Adult Mouse Incisor
7:47
Conclusion
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