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Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

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12.2K Views

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09:36 min

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May 12th, 2014

DOI :

10.3791/51433-v

May 12th, 2014

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Marisa Karow¹, Christian Schichor², Ruth Beckervordersandforth³, Benedikt Berninger¹^,⁴

¹Department of Physiological Genomics, Institute of Physiology, Ludwig Maximilians University Munich, ²Tumor Biology Lab, Neurosurgical Clinic, Ludwig-Maximilians University Munich, ³Institut für Biochemie, Emil-Fischer-Zentrum, Friedrich-Alexander-Universität Erlangen-Nürnberg, ⁴Institute of Physiological Chemistry and Focus Program Translational Neuroscience, Johannes Gutenberg University Mainz

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Lineage reprogramming

Pericyte derived Induced Neurons

Patch clamp Recording

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0:05

Title

1:34

Isolation and Culturing of Adult Human Brain Cells

2:55

In vitro Expansion and Characterization of Adult Human Brain Cells

5:15

Retroviral Transduction of Pericyte-derived Cells and Reprogramming into Induced Neurons

6:41

Results: Reprogramming Adult Brain Cells into Induced Neurons

9:11

Conclusion

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