<h2 class="jove_title"><a href="https://www.jove.com/video/50534">Pre-hospital Thrombolysis: A Manual from Berlin</a></h2>
Martin Ebinger1,2,5, Sascha Lindenlaub1,3,5, Alexander Kunz1,2,5, Michal Rozanski2,5, Carolin Waldschmidt2,5, Joachim E. Weber2,5, Matthias Wendt2,5, Benjamin Winter1,2,5, Philipp A. Kellner4,5, Sabina Kaczmarek4,5, Matthias Endres1,2,5, Heinrich J. Audebert1,2,5
1Center for Stroke Research Berlin (CSB), Charit&#233; - Universit&#228;tsmedizin Berlin, 2Klinik und Hochschulambulanz f&#252;r Neurologie, Charit&#233; - Universit&#228;tsmedizin Berlin, 3Medical School of the Universit&#228;t Hamburg, Universit&#228;tsklinikum Hamburg - Eppendorf, 4Berliner Feuerwehr, 5STEMO-Consortium
Identification of suspected stroke in the dispatch center of the Berlin Fire Brigade prompts the deployment of a CT-equipped ambulance. If ischemic stroke is confirmed and contraindications are excluded pre-hospital thrombolysis is applied.
<h2 class="jove_title"><a href="https://www.jove.com/video/50584">Neural Circuit Recording from an Intact Cockroach Nervous System</a></h2>
Josh S. Titlow1, Zana R. Majeed1,2, H. Bernard Hartman3, Ellen Burns1, Robin L. Cooper1
1<a href='/institutions/NA-north-america/US-united-states/KY-kentucky/159-university-of-kentucky'>Department of Biology, University of Kentucky</a>, 2Department of Biology, University of Salahaddin, 3<a href='/institutions/NA-north-america/US-united-states/OR-oregon/327-university-of-oregon'>Oregon Institute of Marine Biology, University of Oregon</a>
This article describes the cockroach ventral nerve cord dissection and extracellular recordings from the cercal nerve and connectives. Evoked responses are generated by electrical stimulation of the cercal nerve or direct mechanical stimulation of the cerci.
<h2 class="jove_title"><a href="https://www.jove.com/video/50631">Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis</a></h2>
Josh Titlow1, Zana R. Majeed1,2, John G Nicholls3, Robin L. Cooper1
1<a href='/institutions/NA-north-america/US-united-states/KY-kentucky/159-university-of-kentucky'>Department of Biology, University of Kentucky</a>, 2Department of Biology, College of Science, University of Salahaddin, Iraq, 3Department of Neurobiology and Cognitive Neuroscience, SISSA, Italy
This article describes three nervous system preparations using leeches: intracellular recording from neurons in ventral ganglia, culturing neurons from ventral ganglia, and recording from a patch of innervated skin to map sensory fields.
<h2 class="jove_title"><a href="https://www.jove.com/video/50778">Simulation of the Planetary Interior Differentiation Processes in the Laboratory</a></h2>
Yingwei Fei
Geophysical Laboratory, Carnegie Institution of Washington
The high-pressure and high-temperature experiments described here mimic planet interior differentiation processes. The processes are visualized and better understood by high-resolution 3D imaging and quantitative chemical analysis.

november 2013: this month in jove

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/81">November 2013 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments.</a>
In the biomedical sciences, many principles are based on a handful of model organisms&#8212;but experimental findings in traditional models don't always translate to humans or other organisms, nor can they answer all fundamental questions. Therefore, scientists often look to non-traditional models, especially in emerging and multidisciplinary fields.
In <a href="http://www.jove.com/neuro">JoVE Neuroscience</a>, we have two video articles with common animals that are not so common in the lab. One is the freshwater leech, the bloodsucking worm that is firmly embedded in traditional medicine and also appreciated in neuroscience. It has an extremely simple nervous system, which allows neuroscientists to study individual neurons and how their connections influence behavior. <a href="http://www.jove.com/video/50631">Titlow et al.</a> demonstrate how to dissect ventral ganglions and culture isolated neurons (which can retain their electrical characteristics for up to 45 days) and obtain stable intracellular recordings in sensory cells. They might not be very appealing, but leeches are valuable tools for teaching neural anatomy, the principles of intracellular recordings, and intercellular communication.
Another article in <a href="http://www.jove.com/neuro">JoVE Neuroscience</a> features the American cockroach, an unwelcome guest in households all over the world. Anyone who has seen cockroaches in action knows how quickly they can change directions, especially when running away from predators. In fact, of all animals, the cockroach has one of the fastest escape responses, which has been attributed to a reflex circuit that involves two horn-like structures called cerci on the abdomen. Hundreds of tiny sensory hairs on the cerci transform stimuli into neural events, which result in escape behavior. <a href="http://www.jove.com/video/50584">Titlow et al.</a> demonstrate how to prepare the cockroach for electrophysiology and perform neural circuit recordings from intact cockroach nervous systems. These techniques can help identify the neural circuits that underlie certain behavioral responses.
Our featured article from <a href="http://www.jove.com/ctm">Clinical and Translational Medicine</a> deals with acute ischemic stroke, where any delay with emergency treatment can negatively affect the outcome. Therefore, <a href="http://www.jove.com/video/50534">Ebinger et al.</a> present the STEMO, or stroke emergency mobile, a specialized ambulance equipped with a computed tomography scanner and point-of-care lab. This allows vital diagnostics and interventions before the patient gets to the hospital. Preliminary examinations are performed and blood samples taken to determine international normalized ratio (INR), which guides anticoagulation treatment. Advanced diagnostics are also possible through telemedicine, where CT images are transmitted electronically to a neuroradiologist on call. If appropriate, thrombolysis is initiated using recombinant tissue plasminogen activator. The emergency room is notified before transport so everything is in place when the patient arrives. While this pre-hospital concept has not yet been tested in all urban and rural settings, it represents a logical approach for shortening time to treatment for stroke patients.
Many video articles in JoVE show how to create small-scale simulations of larger systems. This month in <a href="http://www.jove.com/ap">JoVE Applied Physics</a>, a very large system is approximated in the lab: the planet Earth, and simulation of its interior differentiation processes to study planetary core formation. <a href="http://www.jove.com/video/50778">Fei</a> does this by mixing olivine silicate, iron powder, and iron sulfide, then loading into a high-pressure cell assembly and heating to 1800&#176;C. The sample is recovered and prepared for 3D imaging. By analyzing high-resolution images and the chemical compositions of the liquid and solid phases, scientists can better understand the process of planetary core formation.
You've just had a sneak peek of a few highlights from the <a href="http://www.jove.com/archive/81">November 2013 issue</a> of <a href="http://www.jove.com/">JoVE</a>. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about November 2013: This Month in JoVE at JoVE.com

November 2013: This Month in JoVE

Here's a look at what's coming up in the <a href="/archive/81">November 2013 issue</a> of JoVE.

Here's a look at what's coming up in the November 2013 issue of JoVE: The Journal of Visualized Experiments.
In the biomedical sciences, many principles ...

Without Section

2.0K Views.  Massachusetts Eye and Ear. Here's a look at what's coming up in the November 2013 issue of JoVE.

Video: November 2013: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Research

JoVE Journal

Biology

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...