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Measuring the Osmotic Water Permeability Coefficient (P_f) of Spherical Cells: Isolated Plant Protoplasts as an Example

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16.0K Views

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14:20 min

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October 8th, 2014

DOI :

10.3791/51652-v

October 8th, 2014

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Arava Shatil-Cohen¹, Hadas Sibony¹, Xavier Draye², François Chaumont³, Nava Moran¹, Menachem Moshelion¹

¹The RH Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, ²Earth and Life Institute, Université catholique de Louvain, ³Institut des Sciences de la Vie, Université catholique de Louvain

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Osmotic Water Permeability

Osmotic Challenge Assay

Pf Measurement

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0:05

Title

2:10

Isolation of Arabidopsis Mesophyll Protoplasts

4:13

The Hypotonic Challenge Assay: Arabidopsis Mesophyll Cell Swelling

6:36

Analysis of Cell Volume Change Using ImageJ

8:12

Modling the Rate of Osmolarity Change in the Experimental Chamber Using ImageJ and the Matlab Program P_fFit

11:11

Determining the P_f Using the Matlab Fitting Program P_fFit

12:30

Results: Determination of the Osmotic Water Permeability Coefficient (P_f) in Plant Protoplasts

13:37

Conclusion

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Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example

Measuring the Osmotic Water Permeability Coefficient (P_f) of Spherical Cells: Isolated Plant Protoplasts as an Example