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In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers

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11.6K Views

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13:10 min

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August 1st, 2014

DOI :

10.3791/51679-v

August 1st, 2014

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Chandirasegaran Massilamany¹, Arunakumar Gangaplara¹, Ting Jia¹, Christian Elowsky², Qingsheng Li³, You Zhou², Jay Reddy¹

¹School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, ²Center for Biotechnology, University of Nebraska, Lincoln, ³Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln

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MHC Class II Dextramers

Autoreactive CD4 T Cells

Experimental Autoimmune Encephalomyelitis EAE

Experimental Autoimmune Myocarditis EAM

Laser Scanning Confocal Microscope LSCM

Myelin Proteolipid Protein PLP 139 151

Cardiac Myosin Heavy Chain Myhc 334 352

Theiler s Murine Encephalomyelitis Virus TMEV 70 86

Bovine Ribonuclease RNase 43 56

In Situ Detection

Quantitative Analysis

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0:05

Title

1:49

In Situ Staining of Cerebral Sections with MHC Class II (IA^s)/PLP 139-151 Dextramers

6:28

Analysis of Dextramer⁺ (dext⁺) CD4⁺ T Cells by Laser Scanning Confocal Microscopy (LSCM)

10:50

Results: In Situ Detection of Antigen-specific Autoreactive CD4 T Cells by Direct Staining with MHC Class II Dextramers

12:29

Conclusion

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