High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

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30.1K Views

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13:13 min

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September 23rd, 2014

DOI :

10.3791/52010-v

September 23rd, 2014

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Subarna Bhattacharya*¹, Paul W. Burridge*², Erin M. Kropp¹, Sandra L. Chuppa¹, Wai-Meng Kwok³, Joseph C. Wu², Kenneth R. Boheler⁴^,⁵, Rebekah L. Gundry¹^,⁶

¹Department of Biochemistry, Medical College of Wisconsin, ²Stanford Cardiovascular Institute, Stanford University School of Medicine, ³Department of Anesthesiology, Medical College of Wisconsin, ⁴Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, ⁵Division of Cardiology, Johns Hopkins University School of Medicine, ⁶Cardiovascular Research Center, Biotechnology and Bioengineering Center, Medical College of Wisconsin

Резюме

The article describes the detailed methodology to efficiently differentiate human pluripotent stem cells into cardiomyocytes by selectively modulating the Wnt pathway, followed by flow cytometry analysis of reference markers to assess homogeneity and identity of the population.

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Human Pluripotent Stem Cells

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