Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease

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10:14 min

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April 19th, 2015

DOI :

10.3791/52451-v

April 19th, 2015

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Ophélie Vacca¹^,²^,³, Brahim El Mathari¹^,²^,³, Marie Darche¹^,²^,³, José-Alain Sahel¹^,²^,³, Alvaro Rendon¹^,²^,³, Deniz Dalkara¹^,²^,³

¹Department of Therapeutics, Institut de la Vision, Sorbonne Universtés, UPMC Univ Paris 06, UMR_S 968, ²INSERM, U968, ³CNRS, UMR_7210

Резюме

To investigate the blood-retinal barrier permeability and the inner limiting membrane integrity in animal models of retinal disease, we used several adeno-associated virus (AAV) variants as tools to label retinal neurons and glia. Virus mediated reporter gene expression is then used as an indicator of retinal barrier permeability.

Смотреть дополнительные видео

Adeno associated Virus

Retinal Barriers

M ller Cells

Blood retinal Barrier

Inner Limiting Membrane

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