Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
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14:09 min
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August 4th, 2015
DOI :
August 4th, 2015
•1Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, 2Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 3Charles Perkins Centre, The University of Sydney, 4Institute of Biophysics, Laboratory of RNA Biology, Chinese Academy of Sciences, 5University of Chinese Academy of Sciences, 6School of Medicine and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University
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0:05
Title
2:28
Human RBCs Isolation, Biotinylation and Osmolarity Adjustment
4:07
Preparation for Micropipettes and a Cell Chamber
6:24
BFP Experiment
10:25
Fluorescence BFP (FBFP) Experiment
11:51
Results: Representative T-cell Receptor and Peptide MHC Binding Kinetics Data & Calcium Fluorescence Data from the FBFP Experiment
13:14
Conclusion
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