A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies
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10:13 min
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November 7th, 2015
DOI :
November 7th, 2015
•Emily J. Chadwick1, David P. Yang1, Mariella G. Filbin2, Emanuele Mazzola3, Yu Sun1, Oded Behar1,4, Maria F. Pazyra-Murphy1, Liliana Goumnerova5, Keith L. Ligon6, Charles D. Stiles1, Rosalind A. Segal1
1Department of Cancer Biology, Dana-Farber Cancer Institute, 2Department of Pediatrics, Children's Hospital, 3Department of Biostatistics & Computational Biology, Dana-Farber Cancer Institute, 4Department of Developmental Biology and Cancer Research, Hebrew University of Jerusalem, 5Department of Neurosurgery, Children's Hospital, 6Center for Molecular Oncologic Pathology, Department of Medical Oncology, Dana-Farber Cancer Institute
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0:05
Title
0:49
Dissection
2:02
Embedding the Brains in Agarose
3:28
Slicing with the Vibratome
5:31
Plating the Slices onto the Inserts
6:53
Plating the Tumor Cells and Staining
8:57
Results: Mouse Medulloblastoma Cells Treated with LDE225 (Sonidegib)
9:37
Conclusion
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