Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
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09:54 min
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November 19th, 2015
DOI :
November 19th, 2015
•Joseph S. Najem1, Myles D. Dunlap2, Anthony Yasmann3, Eric C. Freeman4, John W. Grant5, Sergei Sukharev3, Donald J. Leo4
1Department of Mechanical Engineering, Virginia Polytechnic Institute and State University, 2School of Biomedical Engineering and Sciences, Virginia Polytechnic Institute and State University, 3Department of Biology, University of Maryland, 4College of Engineering, University of Georgia, 5Department of Engineering Sciences and Mechanics, Virginia Polytechnic Institute and State University
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0:05
Title
0:48
Preparation of Liposomes
2:00
Manufacture of the Oil Reservoir and Preparation of Electrodes
4:06
Setting Up the Experiment
6:22
Formation of the Lipid Bilayer
7:49
Results: Bilayer Formation and Activation of MscL Channels in Response to Harmonic Mechanical Stimuli
8:59
Conclusion
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