JoVE Journal

Cancer Research

This content is Open Access.

Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps

Here, we provide a workflow that allows the identification of healthy and pathological cells based on their 3-dimensional shape. We describe the process of using 2D projection outlines based on the 3D surfaces to train a Self-Organizing Map that will provide objective clustering of the investigated cell populations.

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0:04

Title

1:11

Reconstruct the 3D Image

3:13

Transform the 3D Reconstructed Surfaces into 2D Projections

4:28

Find the Periphery and Calculate the Fourier Components using Fiji

5:13

Self-Organizing Maps

6:53

Results: Robustness of SOM Mapping Approach

8:32

Conclusion

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