Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

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2.3K Views

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06:34 min

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March 8th, 2024

DOI :

10.3791/61199-v

March 8th, 2024

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Paulina M. Durczak*¹, Kathryn L. Fair*¹, Nicholas Jinks¹, Sara Cuevas Ocaña¹, Carlos B. Sainz Zuñiga¹, Nicholas R.F. Hannan¹

¹Biodiscovery Institute, Translational Medical Sciences, School of Medicine, University of Nottingham

Резюме

This protocol allows for the differentiation of human pluripotent cells into intestinal organoids. The protocol mimics normal human development by differentiating cells into a population of definitive endoderm, hindgut endoderm and then intestinal epithelium. This makes the protocol suitable for studying both intestinal development as well as disease modelling applications.

Смотреть дополнительные видео

HiPSC

Intestinal Organoids

Developmental Modeling