In this research, we focused on establishing accurate and consistent microhardness testing. Microhardness testing is a traditional method, but fear not, the methodology is not sufficiently described in previous publications. As we demonstrated in the result, provide immediate minimal microhardness reduction depends on the intention sites of the interim, like inner, middle and outer layers.
Our results suggests that the intention site is important to obtain accurate and consistent results. The scientific gap is that the previous studies did not sufficiently describe the microhardness testing methods, especially the variation sites. Due to this, microhardness result were not consistent between studies.
To fill this gap, we established this protocol for accurate and effective evaluation. Our established protocol will advance mineral tissue testing in enamel, denti, and earlier bone in periodontal disease, bone origination, and oral cancer bone invasion models. We will focus on how environmental factors affect dental craniofacial pathophysiology.
For example, chloride and organ rolling, PFAS. PFAS is manmade forever hazardous chemicals. Our microhardness protocol will contribute to evaluating how PFAS affect mechanical properties in mineral tissues in tooth development, periodontal bone resorption, and oral cancer bone invasion.
