Our overarching vision is to better understand and grasp cell communication and cell behavior leading to tissue metastasis and how the cellular processes are misregulated in fed repair. To do so, we are using various models of which one is the Spiny mouse. One of the main issues with this new mouse model is the lack of tools to study it.
Here we provide a new protocol, which contains reliable enzyme digestion and antibody staining. Allowing us to successfully sort fibro-adipogenic progenitors and culture them in vitro for further analysis of their fate. We have tested various enzyme combinations and concentrations, as well as multiple antibodies, and our method provides references for the most reliable and robust protocol.
As we now have a reliable enzymatic digestion, we will continue to test for other markers and antibodies to successfully analyze endothelial cells, pericytes, immune cells, and myogenic cells.
