Culture of myeloid dendritic cells from bone marrow precursors

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22.4K Views

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06:39 min

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July 25th, 2008

DOI :

10.3791/769-v

July 25th, 2008

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Jeanette Boudreau¹^,², Sandeep Koshy²^,³, Derek Cummings², Yonghong Wan²

¹Medical Sciences Program, McMaster University, ²Centre for Gene Therapeutics, McMaster University, ³Department of Chemical Engineering, University of Waterloo

Резюме

This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are shown.

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