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JoVE Journal

Biology

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Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay

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12.8K Views

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13:48 min

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September 7th, 2008

DOI :

10.3791/920-v

September 7th, 2008

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Shaochong Zhang¹, Axel Nohturfft¹^,²

¹Department of Molecular and Cell Biology, Harvard, ²Molecular and Metabolic Signalling Centre, Division of Basic Medical Sciences, St. George's University of London

Резюме

Here, we describe procedures for studying changes in phagocytosis-induced gene expression with a luciferase-based reporter gene approach using the Dual-GloTM Luciferase Assay System from Promega.

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Membrane Biogenesis

Luciferase based Reporter Gene Assay

Lipid Synthesis Pathways

Dual Glo Luciferase Assay System

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