Linearization of the Bradford Protein Assay

97.9K Views

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06:35 min

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April 12th, 2010

DOI :

10.3791/1918-v

April 12th, 2010

•

Orna Ernst¹, Tsaffrir Zor¹

¹Department of Biochemistry, Tel Aviv University

Summary

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Biology

590 Nm

Coomassie Brilliant Blue Assay

Protein Quantification

Cell Lysates

Cellular Fractions

Recombinant Protein Samples

Normalization Of Biochemical Measurements

Nonlinearity

Sensitivity

Accuracy

Standard Assay Conditions

Chapters in this video

0:00

Title

0:43

Introduction

1:12

Linearization of the Bradford Protein Calibration Graph

2:55

Measuring Protein Concentration Using the Improved Bradford Protein Assay

4:59

Improved Bradford Protein Assay Representative Results

5:53

Conclusion

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