A subscription to JoVE is required to view this content.

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

In this article, a simple, quantitative, liquid phase affinity capture assay is presented. It is a reliable technique based on the interaction between magnetic beads and tagged proteins (e.g. nanobodies) on one hand and the affinity between the tagged protein and a second, labeled protein (e.g. poliovirus) on the other.

Chapters in this video

0:05

Title

2:27

Preparation of the Magnetic Beads

3:00

Affinity Capture Assay

4:18

Recycling the Beads

5:44

Interpretation of the Results

6:13

Results: Liquid Phase Affinity Capture Assay using PVSS38C Nanobody

7:36

Conclusion

Related Videos

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More