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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

17.2K Views

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10:58 min

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December 3rd, 2010

DOI :

December 3rd, 2010

•

Pauline Chugh*¹, Kristen Tamburro*¹, Dirk P Dittmer¹

¹Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research, University of North Carolina at Chapel Hill

Summary

We will demonstrate the setup and analysis of pre-microRNA 96-well arrays for QPCR using a robot as well as by hand with a Thermo Scientific Matrix multichannel pipette.

Chapters in this video

0:05

Title

1:21

Introduction

2:00

qPCR Reaction Sample Setup

3:03

Setup of the Pre-miRNA Assay Using the Freedom Tecan Evo Robot

5:17

Setup of the Pre-miRNA Assay Using the Matrix Electronic Multichannel Pipette

8:00

Representative Results for Profiling of Pre-micro RNAs and MicroRNAs using qPCR Arrays

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