JoVE Journal

Neuroscience

This content is Open Access.

Membrane Potentials, Synaptic Responses, Neuronal Circuitry, Neuromodulation and Muscle Histology Using the Crayfish: Student Laboratory Exercises

The experiments demonstrate an easy approach for students to gain experience in examining muscle structure, synaptic responses, the effects of ion gradients and permeability on membrane potentials. Also, a sensory-CNS-motor-muscle circuit is presented to show a means to test effects of compounds on a neuronal circuit.

Chapters in this video

0:00

Title

1:30

Surgical Procedure to Expose the Abdominal Extensor Muscles

3:35

Intracellular Recording from the Abdominal Extensors Muscle Fibers

5:50

Results: Effect of K+ Concentration on Intracellular Recordings of Extensor Muscle Fibers

6:42

Neuroanatomical Observations of Abdominal Extensor Muscles

8:03

Recording Synaptic Responses in Abdominal Extensor Muscles

10:00

Results: Comparing Synaptic Responses of DEL and SEL Muscles

11:19

Intracellular Recording of EPSPs and IPSPs from Superficial Flexor Muscles

12:35

Results: EPSPs of Superficial Flexor Muscle Fibers

12:46

Extracellular Recording from Motor Neurons of the Superficial

14:27

Results: Motor Neuron Response to Sensory Stimulation and Serotonin

15:30

Conclusion

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