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Immunology and Infection

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Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis

12.1K Views

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04:37 min

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February 24th, 2011

DOI :

February 24th, 2011

•

James Navalta¹, Brian McFarlin*², Richard Simpson², Elizabeth Fedor¹, Holly Kell¹, Scott Lyons¹, Scott Arnett¹, Mark Schafer¹

¹Department of Kinesiology, Recreation, and Sport, Western Kentucky University, ²Department of Health and Human Performance, University of Houston

Summary

Exercise is capable of inducing apoptosis in immune cells. There are various measurement limitations, particularly relating to the amount of time required to isolate and treat a blood sample prior to the assessment. Demonstrated is a rapid and minimally invasive procedure for the analysis of exercise-induced lymphocyte apoptosis.

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Finger stick Blood Sampling

Exercise induced Lymphocyte Apoptosis

Measurement Limitations

Isolation And Treatment Of Blood Sample

Assessment Of Cell Death

Effect Of Exercise On The Immune System

Rapid And Minimally Invasive Procedure

Analysis Of Exercise induced Lymphocyte Apoptosis

Incubation Period

Red Blood Cells Lysis

Volume Of Blood Required

Discomfort To Subjects

Chapters in this video

0:01

Title

0:29

Finger-Stick Blood Sampling

1:20

Cell Phenotyping and Apoptosis Staining

3:53

Representative Results

4:05

Conclusion

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