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Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

An efficient procedure to assess the oligomerization propensity of single-pass transmembrane domains (TMDs) is described. Chimeric proteins consisting of the TMD fused to ToxR are expressed in an E. coli reporter strain. TMD-induced oligomerization causes dimerization of ToxR, activation of transcription and production of the reporter protein, -galactosidase.

Chapters in this video

0:05

Title

1:26

Expression of the Chimeric Proteins

2:30

Measurement of β-Galactosidase Activity

3:40

Verification of Protein Expression

4:24

Control for Proper Membrane Insertion

5:34

Representative Results of the ToxR Transcriptional Reporter Assay

6:17

Conclusion

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