JoVE Journal

Immunology and Infection

This content is Open Access.

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

Chapters in this video

0:05

Title

1:39

Making HLA-A2-Ig-based aAPC

2:47

Quality Control of aAPC and Peptide Loading and Storage

3:43

Human CTL Isolation

5:22

In Vitro aAPC-based Culture System

6:25

Expanded CTL Are Polyfunctional - Not Exhausted - after Prolonged Cell Culture and Proliferation

6:49

Conclusion

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