JoVE Journal

Bioengineering

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Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

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10:25 min

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December 9th, 2011

DOI :

December 9th, 2011

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Yoonsuck Choe¹, David Mayerich², Jaerock Kwon³, Daniel E. Miller¹, Chul Sung¹, Ji Ryang Chung¹, Todd Huffman⁴, John Keyser¹, Louise C. Abbott⁵

¹Department of Computer Science and Engineering, Texas A&M University, ²Beckman Institute for Advanced Science and Technology, University of Illinois, ³Department of Electrical and Computer Engineering, Kettering University, ⁴3Scan, ⁵Department of Veterinary Integrative Biosciences, Texas A&M University

Summary

The full process from brain specimen preparation to serial sectioning imaging using the Knife-Edge Scanning Microscope, to data visualization and analysis is described. This technique is currently used to acquire mouse brain data, but it is applicable to other organs, other species.

Chapters in this video

0:05

Title

1:27

Specimen Preparation: Golgi-Cox Method

3:20

Specimen Preparation: Nissl Method

4:39

Specimen Preparation: India Ink Method

5:37

KESM Setup and Imaging

6:38

Image Processing, Data Visualization and Analysis

8:12

Representative Results of KESM

10:00

Conclusion

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