JoVE Journal

Biology

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In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

16.8K Views

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07:22 min

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March 29th, 2012

DOI :

March 29th, 2012

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David R. Hyde¹, Alan R. Godwin², Ryan Thummel³

¹Department of Biological Sciences, Center for Zebrafish Research, University of Notre Dame , ²Department of Microbiology, Immunology, and Pathology, Colorado State University , ³Departments of Anatomy and Cell Biology and Ophthalmology, Wayne State University School of Medicine

Summary

We describe a method to conditionally knockdown the expression of a target protein during adult zebrafish fin regeneration. This technique involves micro-injecting and electroporating antisense oligonucleotide morpholinos into fin tissue, which allows testing the protein’s role in various stages of fin regeneration, including wound healing, blastema formation, and regenerative outgrowth.

Chapters in this video

0:05

Title

1:19

Morpholino Resuspension and Fin Amputation

2:37

Morpholino Injection

4:05

Electroporation

5:14

Analysis

6:17

Representative Fin-outgrowth Results

6:55

Conclusion

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