JoVE Journal

Bioengineering

This content is Open Access.

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Isotachophoresis (ITP) is a robust electrokinetic separation and preconcentration technique with applications ranging from toxin detection to sample preparation. We review the physical principles of ITP and the methodology of applying this technique to two specific example applications: separation and detection of small molecules and purification of nucleic acids from cell culture lysate.

Chapters in this video

0:05

Title

1:39

Physics of ITP

3:20

Device Cleaning and Preparation

4:43

Fluorophore Focusing in Peak Mode ITP

5:30

Extraction and Purification of Nucleic Acids from Cultured E. Coli

7:21

Separation of Amino Acides with Cationic Plateau Mode ITP and Non-Focusing Tracer (NFT) for Visualization

8:33

Representative ITP Results

9:54

Conclusion

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