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Immunology and Infection

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Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

13.7K Views

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09:58 min

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June 5th, 2012

DOI :

June 5th, 2012

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Karen L. Joyce¹, Will Morgan², Robert Greenberg², Meera G. Nair¹

¹Institute of Immunology, Department of Microbiology, Perelman School of Medicine, University of Pennsylvania , ²Pathobiology, School of Veterinary Medicine, University of Pennsylvania

Summary

Schistosoma mansoni eggs are potent stimulators of the T helper type 2 (Th2) immune response, characteristic of parasite infection, asthma and allergic inflammation. This protocol utilizes S. mansoni egg injection to generate a CD4 Th2 cytokine-induced inflammatory response in the lung, characterized by lung granuloma formation around the egg, eosinophilia and macrophage alternative activation.

Chapters in this video

0:05

Title

1:25

Purifying Schistosoma mansoni Eggs

4:00

Intraperitoneal Sensitization with S. mansoni Eggs: Day 0

4:34

Intravenous Challenge with S. mansoni Eggs: Day 14

5:06

Experimental Harvest: Day 22

7:06

Preparation of Recovered Tissues

7:53

Results: Inflammatory Response to S. mansoni Infection

9:31

Conclusion

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