JoVE Journal

Medicine

This content is Open Access.

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Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

28.7K Views

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12:04 min

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August 14th, 2012

DOI :

August 14th, 2012

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Chung Wong¹, Evan Vosburgh¹^,², Arnold J. Levine²^,³, Lei Cong², Eugenia Y. Xu¹^,²

¹Raymond and Beverly Sackler Foundation, ²The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, ³School of Natural Sciences, Institute for Advanced Study, Princeton, New Jersey

Summary

We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy.

Chapters in this video

0:05

Title

2:05

Preparation of 1% Agarose-coated 24-well Plates

3:08

Preparation of Single Cell Suspensions

4:17

Spheroid Culture

5:31

Drug Treatment and Monitoring of 3D Spheroid Cultures

7:22

HistoGel-embedding of the NET 3D Spheroids

9:06

Results: Culture of NET 3D Spheroids and Their Response to Drug Treatment

11:29

Conclusion

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