JoVE Journal

Bioengineering

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10:48

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High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

34.4K Views

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10:47 min

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June 11th, 2013

DOI :

10.3791/50356-v

June 11th, 2013

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Rebecca C. Holmberg¹, Alissa Gindlesperger¹, Tinsley Stokes¹, Dane Brady², Nitu Thakore³, Philip Belgrader³, Christopher G. Cooney³, Darrell P. Chandler⁴

¹Application Development, Akonni Biosystems, Inc., ²Manufacturing, Akonni Biosystems, Inc., ³Engineering, Akonni Biosystems, Inc., ⁴Research & Development, Akonni Biosystems, Inc.

Summary

TruTip is a simple nucleic acid extraction technology whereby a porous, monolithic binding matrix is inserted into a pipette tip. Consequently, the sample preparation format is compatible with most liquid handling instruments, and can be used for many medium to high-throughput clinical applications and sample types.

Explore More Videos

TruTip Technology

Nucleic Acid Extraction

Automated Sample Processing

Clinical Samples

Liquid Handling Robots

Genomic DNA Isolation

Fetal DNA Extraction

Eppendorf EpMotion 5070

High throughput

Porous Monolithic Binding Matrix

Nucleic Acid Binding

Chapters in this video

0:05

Title

2:15

The Hamilton STAR liquid-handling Robot

2:51

Setting Up a 96-well DNA Extraction from Whole Blood

5:02

Sample Lysis and Reagent Distribution

7:31

gDNA Extraction

9:12

Nucleic Acid Extraction, Speed, Fidelity and Purity

10:18

Conclusion

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