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Immunology and Infection

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Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells

16.8K Views

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10:03 min

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August 1st, 2013

DOI :

10.3791/50662-v

August 1st, 2013

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Benson Y.H. Cheng*¹, Emilio Ortiz-Riaño*¹, Juan Carlos de la Torre², Luis Martínez-Sobrido¹

¹Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, ²Departments of Immunology and Microbial Sciences, The Scripps Research Institute

Summary

Rescue of recombinant arenaviruses from cloned cDNAs, an approach referred to as reverse genetics, allows researchers to investigate the role of specific viral gene products, as well as the contribution of their different specific domains and residues, to many different aspects of the biology of arenavirus. Likewise, reverse genetics techniques in FDA-approved cell lines (Vero) for vaccine development provides novel possibilities for the generation of effective and safe vaccines to combat human pathogenic arenaviruses.</

Explore More Videos

Recombinant Arenavirus

Vaccine Development

FDA approved Vero Cells

Arenavirus Reverse Genetics

Cell based Arenavirus Minigenome Systems

Recombinant Infectious Arenaviruses

Trisegmented Arenaviruses

Single cycle Infectious Arenaviruses

Plasmid based Reverse Genetics

Rodent Cell Lines

FDA licensed Vaccine

Vaccine Vectors

Chapters in this video

0:05

Title

1:36

Arenavirus Rescue Transfection

4:41

Confirmation of Recombinant Arenavirus Rescue

7:11

r3LCMV and r3Candid#1 Viral Rescue

8:06

Results: Arenavirus in Vero Cells

9:30

Conclusion

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