JoVE Journal
Immunology and Infection
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Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
17.1K Views
•
11:22 min
•
August 23rd, 2013
DOI :
August 23rd, 2013
•Chapters in this video
0:05
Title
1:48
Plating and Maintenance of Human Microvascular Endothelial Cells (HMVEC)
2:33
Neutrophil Isolation using Polymorphprep
4:31
Neutrophil Labeling with Calcein AM
5:47
Neutrophil Binding Assay
7:43
Results: Measuring Neutrophil Adhesion to Activated Primary HMVEC under Static Conditions
10:57
Conclusion
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ISSN 2689-3649
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