JoVE Journal

Chemistry

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Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

We present a method for rapid, reversible immobilization of small molecules and functionalized nanoparticle assemblies for Surface Plasmon Resonance (SPR) studies, using sequential on-chip bioorthogonal cycloaddition chemistry and antibody-antigen capture.

Chapters in this video

0:05

Title

1:48

Preparation of GST and Nanoparticle Conjugates

3:48

Surface Preparation

5:07

Monitoring On-chip Capture-cycloaddition of Functionalized Molecules

6:26

Monitoring Immobilization Density and Determination of Cycloaddition Rates

8:27

Measuring the Binding of FKBP12 to Immobilized AP1497

10:49

Results: Real-time Monitoring of On-chip Small Molecule Immobilization by Surface Plasmon Resonance

13:51

Conclusion

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