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Bioengineering

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Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

11.2K Views

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10:13 min

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June 3rd, 2014

DOI :

10.3791/51215-v

June 3rd, 2014

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Caroline N. Jones*¹^,²^,³, Anh N. Hoang*¹^,²^,³, Laurie Dimisko¹, Bashar Hamza¹, Joseph Martel¹^,⁴, Daniel Irimia¹^,²^,³

¹The BioMEMS Resource Center, Department of Surgery, Massachusetts General Hospital, ²Harvard Medical School, ³Shriners Burns Hospital, ⁴Harvard University School of Engineering and Applied Sciences

Summary

This protocol details an assay designed to measure human neutrophil chemotaxis from one droplet of whole blood with robust reproducibility. This approach circumvents the need for neutrophil separation and requires only a few minutes of assay preparation time. The microfluidic chip enables the repeated measure of neutrophil chemotaxis over time in infants or small mammals, where sample volume is limited.

Explore More Videos

Microfluidic Platform

Neutrophil Chemotaxis

Unprocessed Whole Blood

Neutrophil Migration

Chemoattractant

Red Blood Cell Filtration

Purified Neutrophils

Chapters in this video

0:05

Title

2:22

Microfluidic Device Fabrication

4:19

Microfluidic Assay Preparation

6:11

Sample Preparation: Human Neutrophils From Capillary Blood

6:49

Microscopy and Image Analysis for Neutrophil Chemotaxis Measurements

7:53

Results: Neutrophils Chemotaxis Assay From Whole Blood

8:55

Conclusion

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