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A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

35.3K Views

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10:56 min

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May 30th, 2014

DOI :

10.3791/51441-v

May 30th, 2014

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Zachary J. Storms¹, Elliot Cameron¹, Hector de la Hoz Siegler¹^,², William C. McCaffrey¹

¹Department of Chemical and Materials Engineering, University of Alberta, ²Department of Chemical and Petroleum Engineering, University of Calgary

Summary

A simple protocol to determine the neutral lipid content of algal cells using a Nile Red staining procedure is described. This time-saving technique offers an alternative to traditional gravimetric-based lipid quantification protocols. It has been designed for the specific application of monitoring bioprocess performance.

Explore More Videos

Auxenochlorella Protothecoides

Bioprocess Monitoring

Gravimetric Methods

Cell Membrane Permeabilization

Chapters in this video

0:05

Title

1:52

Isolation of Dry Algal Biomass to be Used as Standards for Fluorescence Readings

2:52

Gravimetric Quantification of Neutral Lipids by Hexane Extraction

4:48

Fluorometric Quantification of Neutral Lipids Using Nile Red

6:42

Fluorescence Microscopy Technique

8:04

Results: Images and Calibration Curves of A. protothecoides Stained with Nile Red

10:18

Conclusion

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