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Neuroscience

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Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology

21.3K Views

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13:58 min

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June 25th, 2014

DOI :

10.3791/51749-v

June 25th, 2014

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Hao Chang¹, Yanshu Wang¹, Hao Wu¹, Jeremy Nathans¹^,²^,³

¹Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, ²Department of Neuroscience, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, ³Department of Ophthalmology, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine

Summary

Mammalian skin contains a diverse array of structures - such as hair follicles and nerve endings - that exhibit distinctive patterns of spatial organization. Analyzing skin as a flat mount takes advantage of the 2-dimensional geometry of this tissue to produce full-thickness high-resolution images of skin structures.

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Skin Structures

2D Visualization

Optical Sectioning

Tissue Heterogeneity

Chapters in this video

0:06

Title

2:01

Dorsal Skin for Immunostaining and Melanin Imaging

5:40

Foot Skin for the Analysis of Hair Follicle Patterning

6:42

Foot Skin for Visualizing Sebaceous Glands

7:23

Tail Skin Preparation for the Analysis of Melanin Distribution, Hair Follicle Orientation, and Sebaceous Gland Visualization

8:54

Imaging Hair Follicle Orientation

9:52

Imaging and Tracing Axon Arbors

10:55

Results: Geometry of Various Skin Structures Are Easily Visualized in Skin Flatmounts

12:51

Conclusion

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