JoVE Journal

Engineering

A subscription to JoVE is required to view this content.

live

Speed

×

MEDIA_ELEMENT_ERROR: Format error

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

A module for single plane illumination microscopy (SPIM) is described which is easily adapted to an inverted wide-field microscope and optimized for 3-dimensional cell cultures. The sample is located within a rectangular capillary, and via a microfluidic system fluorescent dyes, pharmaceutical agents or drugs can be applied in small quantities.

Chapters in this video

0:05

Title

2:03

Cell Spheroids Incubated with a Chemotherapeutic Drug

2:55

Light Sheet Adjustment

4:09

Cell Spheroid Application and Microscope Feed Synchronization

5:31

Measurement in Dynamic Liquid Environment and Data Acquisition and Analysis

7:00

Results: Imaging of Cell Spheroids Incubated with the Cytostatic Drug Doxorubicin

8:07

Conclusion

Related Videos

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More