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Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement

11.1K Views

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09:50 min

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October 1st, 2014

DOI :

10.3791/52079-v

October 1st, 2014

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Alban Longchamp*¹, Florent Allagnat*², Xavier Berard³, Florian Alonso², Jacques-Antoine Haefliger², Sébastien Deglise*⁴, Jean-Marc Corpataux*⁴

¹Department of Surgery, Brigham and Women's Hospital/Harvard Medical School, ²Laboratory of Experimental Medicine, Department of Medicine, CHUV University Hospital, ³Department of Vascular Surgery, Pellegrin Hospital, University of Bordeaux, ⁴Department of Thoracic and Vascular Surgery, CHUV University Hospital

Summary

The mechanisms leading to the development of intimal hyperplasia (IH) and vein graft failure are still poorly understood. This study describes an ex vivo system to perfuse human veins under controlled flow and pressure. Furthermore the efficiency of external mesh reinforcement to limit the development of IH was evaluated.

Explore More Videos

Ex Vivo Perfusion

External Reinforcement

Intimal Hyperplasia

Vascular Remodeling

Arterial Hemodynamics

Chapters in this video

0:05

Title

2:02

Harvesting Human Great Saphenous Veins

3:14

The Ex Vivo Perfusion System (EVPS)

3:58

EVPS Assembly

6:20

Perfusion

8:13

Results: Histomorphometry

9:12

Conclusion

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