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Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

12.3K Views

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12:00 min

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January 22nd, 2015

DOI :

10.3791/52323-v

January 22nd, 2015

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Andrew R. Bader¹, Tina Li¹, Weiping Wang², Daniel S. Kohane², Joseph Loscalzo¹, Ying-Yi Zhang¹

¹Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, ²Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care, Boston Children's Hospital

Summary

The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.

Explore More Videos

Dextran Sulfate

Polyelectrolyte Complexes

Protein Delivery

Stem Cell Homing

Chapters in this video

0:05

Title

1:22

Preparation of Stromal Cell-derived Factor-1α (SDF-1α) Glycan Nanoparticles

3:34

Measurement of Particle Size and Zeta Potential

5:07

Quantification of SDF-1α in the Particles

6:26

In Vitro Release Assay

7:38

Migration Assay

9:19

Results: Analysis of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

11:23

Conclusion

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