JoVE Journal

Developmental Biology

This content is Open Access.

An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System

A protocol to generate a co-culture system consisting of neurons derived from induced pluripotent stem cells (iPSCs), primary cortical neurons and astrocytes is described. This co-culture system allows detection of the formation of synaptic contacts and circuits between new, iPSC-derived neurons and pre-existing cortical neurons expressing channelrhodopsin-2.

Chapters in this video

0:05

Title

2:12

Cortical Tissue Dissociation

3:52

Electroporation and Plating of Primary Cortical Neurons

5:14

Differentiation of Human Neural Progenitor Cells into Neurons in Co-culture

6:55

Electrophysiological Recordings of Induced Pluripotent Stem Cell-derived Neurons

9:05

Results: Co-culture System to Assess Neuronal Connection Formation

10:53

Conclusion

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