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Developmental Biology

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Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing

Zebrafish keratocytes migrate in cell sheets from explants and provide an in vitro model for the study of the mechanisms of collective cell migration in the context of epithelial wound healing. These protocols detail an effective way to establish primary explant cultures for use in collective cell migration assays.

Chapters in this video

0:05

Title

1:55

Preparation of Anesthesia, Equipment, and Media

2:55

Establish Explant Cultures

4:40

Brightfield and Video Microscopy

5:20

Fixation for Immunofluorescence Microscopy

7:02

Results: Cell Migration in Zebrafish Keratocytes

8:40

Conclusion

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