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Medicine

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Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

14.3K Views

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07:36 min

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May 1st, 2015

DOI :

10.3791/52691-v

May 1st, 2015

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Zerina Lokmic¹^,², Elizabeth S. Ng¹^,³, Matthew Burton¹, Edouard G. Stanley¹^,²^,³, Anthony J. Penington¹^,², Andrew G. Elefanty¹^,²^,³

¹Murdoch Childrens Research Institute, The Royal Childrens Hospital, ²Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, ³Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton

Summary

The goal of this protocol is to isolate lymphatic endothelial cells lining human lymphatic malformation cyst-like vessels and foreskins using fluorescence-activated cell sorting (FACS). Subsequent cell culturing and expansion of these cells permits a new level of experimental sophistication for genetic, proteomic, functional and cell differentiation studies.

Explore More Videos

Lymphatic Endothelial Cells

Lymphatic System Disorders

Primary Lymphedema

Lymphatic Malformations

Lymphatic Tumors

Fluorescence activated Cell Sorting

Chapters in this video

0:05

Title

1:14

Foreskin and Lymphatic Malformation Cell Suspension Preparation

4:01

Antibody Staining of Lymphatic Endothelial Cell (LEC) Surface Markers

5:49

Results: Representative Foreskin and Lymphatic Malformation LECs

6:53

Conclusion

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