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Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

8.5K Views

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11:43 min

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April 3rd, 2015

DOI :

10.3791/52735-v

April 3rd, 2015

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Nicholas Agung Kurniawan¹, Parthiv Kant Chaudhuri², Chwee Teck Lim²^,³

¹Systems Biophysics Department, FOM Institute AMOLF, ²Mechanobiology Institute, National University of Singapore, ³Department of Biomedical Engineering, National University of Singapore

Summary

The mechanical properties and microstructure of the extracellular matrix strongly affect 3D migration of cells. An in vitro method to study the spatiotemporal cell migration behavior in biophysically variable environments, at both population and individual cell levels, is described.

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3D Cell Migration

Extracellular Matrix

Breast Cancer Cells

Live cell Imaging

Biophysical Microenvironment

Chapters in this video

0:05

Title

1:22

Harvesting Cells

2:32

Preparing the Collagen Solutions

3:52

Formation of Concentric Gel Cultures

6:54

Live-Cell Imaging of Concentric Gel Cultures

8:16

Tracking Cell Movement

9:38

Results: Cell Movement Analysis

11:03

Conclusion

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